Fig. 6

Mechanisms of PAK on lipid level reduction. A Under PAK treatment (1 μmol/kg, every 3 days, i.v injection) and high-fat diet (HFD), tumor volumes were measured every three days. B, C Tumor tissues were collected on day 18 after treatment, and tumor images and weights were recorded. D Immunohistochemical analysis was conducted to measure the expressions of FASN, SCD1, Vimentin, and Ki67 in tumor tissue. Frozen tumor tissue sections were conducted with Nile Red and TUNEL staining. Scale bars, 50 μm. E Quantitative analysis of SCD1, FASN, Vimentin level and Nile Red staining. F Quantitative analysis of Ki67 level. G Quantitative analysis of TUNEL staining. Data are mean ± SD (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significant