Fig. 5

Antitumor effects of PAK can be reversed by SREBP-1 overexpression. After overexpression of SREBP-1 for 24 h, MDA-MB-231 cells were treated with PBS or PAK. A Western blot detection of related genes after overexpression of SREBP-1. B Nile Red fluorescence intensity distribution in MDA-MB-231 cells treated with PAK or/and OE-SREBP-1 was measured. C CCK8 assay was applied to measure the relative cell viability of each group. D Transwell assay was conducted. The photographs show images of cells on the underlayer of the membrane. Scale bar: 100 μm. E Wound healing experiment showed the condition of migrating cells. Scale bar: 100 μm. F MDA-MB-231 cells were treated with PBS or PAK for 24 h. PA&OA or vehicle was added into the cell culture medium additionally. CCK8 assay was used to determine relative cell viability. Data shown are mean ± SD (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significant