Fig. 4

Interaction of PAK with SREBP1 pathway mRNA. A RNA immunoprecipitation was used to detect the binding of protein and mRNA. B Decay of mRNA was monitored in MDA-MB-231 cells treated with Actinomycin D and PAK. The decrease in mRNA levels was determined by qPCR analysis for each time point. Data were fitted by Nonlinear regression. Data shown are mean ± SD (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significant