Fig. 3

Transcriptomic analysis after PAK treatment in MDA-MB-231 cells. Cells were obtained for RNA-seq analysis after treatment with PBS or PAK for 24 h. A Analysis software generated the volcano plot of differentially expressed genes, where the red dots are up-regulated and the blue dots are down-regulated. B KEGG pathways highly associated with changes resulting from PAK treatment. C Gene expression calculation uses HiSeq Analysis Software (v0.9), which uses FPKM [36] method to calculate gene expression. D Relative mRNA expression of SREBP1 target genes from MDA-MB-231 cells treated with PBS or PAK for 24 h was measured. Data were normalized with β-actin. E, F Protein levels were measured by Western blotting in MDA-MB-231 cells treated with PBS or PAK for 24 h. G Nile Red fluorescence intensity in MDA-MB-231 cells treated with PBS or PAK for 24 h was measured. Data shown are mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; versus PBS-treated group