Fig. 2

PAK exhibits antitumor effects against TNBC in vivo. A Under the treatment of PAK (1 μmol/kg, every 3 days, i.v injection), tumor volumes were measured every three days. B, C Tumor tissues were collected on day 18 after treatment, and tumor images and weights were recorded. D Body weight of each mouse was measured every three days. E Immunohistochemical analysis was conducted to measure the Ki67 level in tumor tissue. Frozen tumor tissue sections were conducted with TUNEL apoptosis assay. Scale bars, 50 μm. F Quantitative analysis of Ki67 level and TUNEL staining. Data are mean ± SD (n = 5). *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significant; vs PBS-treated group