Fig. 7

Silencing of PNPLA8 decreases cellular arachidonic, eicosanoids and secreted PGE2 levels. A PCA score scatter plot of eicosanoids in SUM159PT and Hs578T cell lines treated with PNPLA8 siRNA or scrambled siRNA as control. The x-axis and y-axis indicate the first principal component and second principal component, respectively. B OPLS-DA score scatter plot of eicosanoids in SUM159PT and Hs578T cell lines treated with PNPLA8 siRNA or scrambled siRNA as control. C Heatmap of log normalized abundance of eicosanoids in SUM159PT and Hs578T cell lines treated with PNPLA8 siRNA or scrambled siRNA as control. Each cell line is represented by 5 biological replicates. D Venn-diagram indicating the number of eicosanoids which were consistently increased or decreased following PNPLA8 siRNA treatment in both SUM159PT and Hs578T cell lines. E Relative abundance of eicosanoids from the Venn-diagram in (D). F PGE2 levels in culture media of SUM159PT and Hs578T cell lines treated with PNPLA8 siRNA or scrambled siRNA as control. Each cell line is represented by 3 biological replicates. *p < 0.05, **p < 0.001, ***p < 0.0001