Fig. 6

Silencing of PNPLA8 disrupts phospholipid remodeling. A PCA score scatter plot of lipid features in SUM159PT and Hs578T cell lines treated with PNPLA8 siRNA or scrambled siRNA as control. The x-axis and y-axis indicate the first principal component and second principal component, respectively. B OPLS-DA score scatter plot of ion features in SUM159PT and Hs578T cell lines treated with PNPLA8 siRNA or scrambled siRNA as control. C Heatmap of log normalized abundance of lipid classes in PNPLA8 siRNA-treated Hs578T cells versus scrambled siRNA-treated Hs578T control cells. Each siRNA treatment group is represented by 5 biological replicates. Blue boxes outline phospholipids decreased in PNPLA8 siRNA-treated Hs578T cells. Red boxes outline phospholipids increased in PNPLA8 siRNA-treated Hs578T cells. D Heatmap of log normalized abundance of lipid classes in PNPLA8 siRNA-treated SUM159PT cells versus scrambled siRNA-treated SUM159PT control cells. Each siRNA treatment group is represented by 5 biological replicates. Blue boxes outline phospholipids decreased in PNPLA8 siRNA-treated SUM159PT cells. Red boxes outline phospholipids increased in PNPLA8 siRNA-treated SUM159PT cells. E–F Percentage of significantly upregulated, downregulated, and unchanged species of each lipid classe in PNPLA8 siRNA-treated Hs578T (E) and SUM159PT (F) cells versus their corresponding scrambled siRNA-treated control cells. p values < 0.05 were considered significant. G Venn-diagram showing the lipids which were consistently increased or decreased in PNPLA8 siRNA-treated SUM159PT (purple circle) and Hs578T cell lines (pink circle) compared with scrambled siRNA-treated SUM159PT and Hs578T cell lines. H Relative abundance of the 15 lipids which increased following PNPLA8 siRNA treatment as obtained from the Venn-diagram in (G). I Relative abundance of the 5 lipids which decreased following PNPLA8 siRNA treatment as obtained from the Venn-diagram in (G). *p < 0.05, **p < 0.001, ***p < 0.0001