Fig. 3

PNPLA8 expression levels in breast cancer cell lines and its correlation with breast cancer patient survival. A Heatmap of log normalized mRNA level of key genes involved in phospholipid metabolism in our panel of breast epithelial (MCF10A, MCF12A) and breast cancer cell lines (MCF7, BT474, SKBR3, MDA-MB-231, MDA-MB-468, SUM149PT, SUM159PT, Hs578T). Each cell line is represented by 3 biological replicates. The yellow box outlines PNPLA8 overexpression in breast cancer cells. B Protein expression level of PNPLA8 in a panel of breast cancer cell lines. GAPDH was used as a control to confirm equal loading of protein. C Relapse-free survival rates of breast cancer patients with low (n = 1019) or high (n = 1013) expression levels of PNPLA8. The survival curve was obtained from KM-plotter