Fig. 2

Critical lipid species dysregulated in triple-negative breast cancer cell lines. A Volcano plot of fold changes of individual lipids in TNBC cell lines (MDA-MB-231, SUM159PT, Hs578T, MDA-MB-468, SUM149PT) compared with immortal human mammary epithelial cell lines (MCF10A, MCF12A). Each sphere represents one ion feature. Blue spheres represent lipids with p < 0.05. Red spheres represent lipids with p < 0.05 and fold change (FC) > 2 or (FC) < 0.5. B S-plot comparing TNBC cell lines to immortal human mammary epithelial cell lines. Each green sphere represents one ion feature. Red and blue spheres represent significant upregulated or downregulated lipids, respectively, in TNBC cell lines compared with control cell lines. C–D Relative abundance of the top 10 of both upregulated (C) and downregulated (D) lipids selected by S-plot significance shown in (B) in TNBC cell lines compared with two immortal human mammary epithelial cell lines. PC/PE O-represents ether linked phospholipids. For hexosylceramide (HexCer)/Hex2Cer/SM x:y;z, x represents fatty acid carbon chain length, y represents number of double bonds on fatty acid moiety, and z represents oxygen molecules present on fatty acid moiety. ***p < 0.0001