Fig. 8

Fibroblasts lacking EDA fibronectin prevent metastasis formation. A AT3 coinjected with EDA+ MEFs generate bigger tumors than those with EDA– MEFs. Orthotopic tumors were generated in NOD-SCID gamma mice (Additional file 1: Fig. S10A). After resection, the three main dimensions of primary tumors were measured to calculate their volumes. The volume of each tumor relative to the average volume of EDA– MEF co-injected tumors is plotted. B Lung metastasis are absent in EDA– co-injected tumors. Lungs from injected mice in A were extracted 7 weeks after resection of primary tumors, fixed in 4% PFA and embedded in paraffin. H&E staining of lung slices to visualize metastasis are shown in the Additional file 1: Fig. S10B. The plot shows a quantification of the presence of metastatic foci obtained from lung H&E staining. Lungs with at least one metastasis are indicated as positive