Fig. 2

Elevated percentages of EDA+ fibronectin correlate with high SNAIL1 levels in advanced human tumors. A Breast adenocarcinoma; C kidney renal clear cell carcinoma; D skin cutaneous melanoma; and E lung adenocarcinoma. The percentage of EDA+ fibronectin RNA and the SNAIL1 protein level in each specimen were obtained from the TSVdb and cBioPortal databases, respectively. Tumors were classified according to the levels (low or high) of SNAIL1 and EDA+ fibronectin (see Materials and Methods), and the percentages of high or low EDA+ fibronectin tumors were plotted for each SNAIL1-expressing category. Tumors at the initial (I and II) or advanced (III and IV) stages were analyzed separately (see Materials and Methods). Numbers within the bars indicate the percentage of tumors with high levels of EDA+ fibronectin. When available, data for normal tissue (NT) are also shown. n, number of tumors per group. B Relative protein levels of EDA+ fibronectin and SNAIL1 in PDXs. EDA+ fibronectin and SNAIL1 levels in 29 PDX protein extracts from HER2+ or triple-negative breast neoplasms were densitometrically estimated from Western blots (see Materials and Methods). PDXs were classified according to their levels (low or high) of SNAIL1 and EDA+ fibronectin (see Materials and Methods) and analyzed as in A. n, number of PDXs