Fig. 6

IL-6 restores breast cancer proliferation and lung metastasis inhibited by adipocytes overexpressing Grem2. A–C After differentiation of 3T3-L1-mock or 3T3-L1-Grem2 into adipocytes for 9 days, adipocytes-mock or adipocytes-Grem2 were mixed with MTV/TM-011 cells and injected into mammary fat pads of mice. Representative images of primary tumors (A), tumor volume (B), and lung metastatic foci (C). Black arrowheads indicate prominent lung metastatic foci. D Representative immunofluorescence images of vimentin, slug, and twist1 in tumors. Scale bar = 100 μm. Two-way ANOVA. *, p < 0.05; **, p < 0.01; ns, not significant