Fig. 8

SND1 promotes the metastatic phenotype of TNBC cells via DNMT3A. A Scratch wound assay results from MDA-MB-231 and BT549 cells of scramble shRNA (scramble), two SND1 knocking down (SND1-sh1; SND1-sh2) and two DNMT3A rescue expression (SND1-sh1 + DNMT3A; SND1-sh2 + DNMT3A res; n = 3; **, P < 0.01). B Transwell results of aforementioned BT549 and MDA-MB-231 cells (n = 3; **, P < 0.01). C Tumour cell pulmonary metastasis assay results showed the metastasis potential of MDA-MB-231 transfected with scramble control (scramble), SND1-knockdown (SND1-sh1; SND1-sh2), DNMT3A rescue expression (SND1-sh1 + DNMT3A; SND1-sh2 + DNMT3A). Representative bioluminescence images of pulmonary metastasis were presented (left part). The relative optical intensities of photon flux were calculated (right part; n = 8; ***, P < 0.001). D K-M assay results from that different groups of mice bearing MDA-MB-231 cells of scramble control (scramble), SND1-knockdown (SND1-sh1; SND1-sh2), DNMT3A rescue expression (SND1-sh1 + DNMT3A; SND1-sh2 + DNMT3A; n = 8; ***, P < 0.001). E Schematic diagram of SND1 function on DNMT3A and CDH1