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Fig. 7 | Breast Cancer Research

Fig. 7

From: The chromatin architectural regulator SND1 mediates metastasis in triple-negative breast cancer by promoting CDH1 gene methylation

Fig. 7

DNMT3A promotes methylation of CDH1 promoter and inhibits CDH1 expression. A PCR tests expression of SND1, DNMT3A and CDH1 in BT549 and MDA-MB-231 of scramble shRNA (scramble), two SND1 knocking down (SND1-sh1/sh2) and two DNMT3A rescue expression (SND1-sh1 + DNMT3A; SND1-sh2 + DNMT3A res; n = 3; **, P < 0.01, ***, P < 0.001). B MeDIP analysis of methylation level on CHD1 promoter in aforementioned BT549 and MDA-MB-231 cells (n = 3; *, P < 0.05, **, P < 0.01). C Western results of SND1, DNMT3A and CDH1 expressions in aforementioned MDA-MB-231 and BT549 cell. D Immunofluorescence results of DNMT3A and CDH1 expressions in aforementioned MDA-MB-231 and BT549 cell. (E) Compare positive stain per cent of DNMT3A and CDH1 in aforementioned MDA-MB-231 and BT549 cell (n = 5; **, P < 0.01)

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Breast Cancer Research

ISSN: 1465-542X

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