Fig. 6

SND1 represses the expression of CDH1 via inducing CDH1 promoter methylation. A qRT-PCR results of CDH1 and DNMT3A levels in BT549 and MDA-MB-231 cells of scramble group (scramble), two SND1-knocking down groups (SND1-sh1; SND1-sh2) and two SND1 rescue expression groups (SND1-sh1 + SND1 res; SND1-sh2 + SND1 res; n = 3; **, P < 0.01, ***, P < 0.001). B CDH1 Western blotting data from MDA-MB-231 and BT549 cell treated with different amounts of 5-aza. C MeDIP results of methylation level on CHD1 promoter in aforementioned treated BT549 and MDA-MB-231 cells (scramble; SND1-sh1; SND1-sh2; SND1-sh1 + SND1 res; SND1-sh2 + SND1 res; n = 3; **, P < 0.01). D ChIP results of DNMT3A binding condition on CDH1 promoter in aforementioned MDA-MB-231 and BT549 cells (n = 3; **, P < 0.01). E Bisulfite sequencing PCR results of methylation level on CHD1 promoter in aforementioned MDA-MB-231 and BT549 cells. F Western blotting data of SND1, DNMT3A and CDH1 levels in aforementioned MDA-MB-231 and BT549 cells