Fig. 5

SND1 enhances transcription of DNMT3A by promoting chromatin long-range interaction in DNMT3A prompter. A, B ChIP assay data showed the histone acetylation on H3K9ac (A) and H3K27ac (B) at DNMT3A promoter after SND1 knocking down. C The first anchor was SND1 recognition regions R1, 3C data showed the DNA cross-link among R1 to R2 and the TSS without SND1 knockdown. The SND1 depletion inhibited the forming of R1/TSS cross-link but not the forming of R1/R2 cross-link. The vertical lines displayed the restriction enzyme cleavage sites and the arrows represented PCR primers. D The second anchor was R2, and the cross-links among R2 to R1 and TSS were displayed by 3C. E TSS was the final anchor, and the cross-links among TSS to R1 and R2 were shown. F The image-schematic structure presented the mechanism of SND1 promoting DNMT3A transcription. All data were shown in mean ± SD. n = 3, **, P < 0.01