Fig. 1

Manhattan plot of association results between chemotherapy and DNA methylation for 588,798 probes (125 breast cancer patients with pre- and post-chemotherapy paired samples), adjusted for the proportion of monocytes (limma design matrix ~ 0 + time + serial + monocytes). The term “time” refers to the specific time at which the sample was collected (i.e. pre- or post-chemotherapy). The term “serial” denotes the patient’s unique study ID. Using a Bonferroni threshold p-value < 8.49e-8, represented by a red line, a total of 1568 associations were found to be statistically significant (Additional file 3)