Fig. 6
From: mTOR inhibition abrogates human mammary stem cells and early breast cancer progression markers
Effect of sirolimus treatment on proliferation and cell cycle markers A Ki67 staining by IHC and quantitative analysis of positively stained nuclei of pre- and post-sirolimus treatment in normal (n=12) and DCIS tissue (n=12). B p21 nuclear staining by IHC and quantitative expression analysis in normal (n=9) and DCIS tissues (n=5) from pre- and post-treatment samples. Significance was evaluated by paired t-test for Ki67 and 2-way ANOVA for p21. Scale bar, 100 μm.