Fig. 3

Effect of sirolimus treatment on SFE of BM and LP cells. A Scatter plots of the SFE of FACS-sorted BM cells of control (n = 18) and treated (n = 22) patient-derived tissue for all groups with unpaired t-test. Pre-menopausal B and postmenopausal patients C are compared between treatment (n = 5, n = 17) and control groups (n = 8, n = 10). D Scatter plots of sorted LP cells are also shown for control (n = 18) and treated (n = 22) patient groups including pre-menopausal E and postmenopausal patients F comparisons between treatment (n = 5, n = 17) and control groups (n = 8, n = 10). G Serial passaging of LP cells in 3D organoid culture for comparison of self-renewal capacity between control (n = 11) and treatment groups (n = 5) for a duration of 5 passages and differences were evaluated by 2-way ANOVA