Fig. 6

MiR-4649-5p upregulation and PIP5K1C inhibition reduce AKT phosphorylation, potentiating growth reduction by AKT inhibitor capivasertib. A Representative Western Blot of SUM159 cells treated with increasing concentrations of the pharmacologic PIP5K1C inhibitor UNC3230 or DMSO as vehicle control, for either 24 or 48 h (on the left), and the quantification of the detected Pospho-AKT (Ser473) normalized to the housekeeper Cofilin and total AKT (on the right). B Representative Western Blot of SUM159 treated with 10 nM miR-4649-5p mimic or mimic control in combination with 10 μM UNC3230 or DMSO as vehicle control for 24 h (on the left), and the quantification of the detected Pospho-AKT (Ser473) normalized to the housekeeper Cofilin and total AKT (on the right). C SUM159 cells were transiently transfected with miR-4649-5p or the mimic control and treated with 0.5 μM of the AKT inhibitor capivasertib or DMSO as vehicle control. Effects on cell growth were observed in a WST-1 assay (n = 6; mean ± SD; *p ≤ 0.05, ***p ≤ 0.005, compared by One-way ANOVA and Tukey multiple comparison test)