Fig. 5

AGE stimulates fibroblasts to promote cellular migration and invasion in a RAGE dependent manner. A RAGE mRNA stromal levels in normal and invasive breast cancer cases from Oncomine. B ELISA (left panel) and qPCR (right panel) analysis of RAGE levels in primary mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. C qPCR analysis of mouse mammary fibroblasts isolated from 7-week old mice fed regular or high AGE diet. D Schematic representation of two compartment transwell co-culture assay. Transwell migration assay of HC11 E and Met1 F cells co-cultured with either no fibroblasts or fibroblasts isolated from mice fed a regular (REG) or high AGE (HIGH) diet. Fibroblasts in the lower chamber were also either untreated or treated with AGE ex vivo. Transwell migration (G) and invasion (H) of Met1 epithelial cells co-cultured with fibroblasts isolated from RAGE +/+ (regular n = 2; high AGE n = 2) or RAGE-/- (regular n = 2; high AGE n = 2) mice, and either untreated or treated with ex vivo AGE. Technical Replicates (n = 2); Biological Replicates (n = 3). Values are mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001