Fig. 4

High AGE consumption induces atypical ductal hyperplasia. A Representative images of ducts representing low grade MIN from 12-week old mice fed a high AGE diet stained with H&E (i) and Ki67 (ii). B Quantitation of the percentage of abnormal ducts observed within the 12-week mammary glands of mice fed the different diets. (C) Quantitation of Ki67 in ducts of 7- and 12-week old mice fed regular, low AGE and high AGE diet. Quantitation of the percentage of (D) abnormal ducts and (E) Ki67 observed within the mammary glands of 7-month old mice fed the regular and high AGE diet. F Schematic representation of experimental design for AGE diet switch experiments on mammary gland development. G Quantitation of Ki67 in ducts of 12-week old mice fed switch diets. Mouse numbers: 7 week—regular (n = 3), low AGE (n = 4), high AGE (n = 4); 12 week—regular (n = 3), low AGE (n = 4), high AGE (n = 3) REG-HIGH (n = 4), HIGH-REG (n = 5); 7 month – regular (n = 3); high AGE (n = 4). Values are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.005