Fig. 3

High AGE consumption results in increased stromal recruitment to TEB structures. A Representative H&E images of TEB structures from 7-week old mice fed a regular or high AGE diet. Inset shows increased magnification of histologically identified and recruitment of macrophages (arrowheads) and fibroblasts (arrow) around TEB structure. Scale bar = 200 µM. B Quantitation of the ratio of recruited stroma to TEB structure in 7-week old mice fed a regular and high AGE diet. C IHC staining of α-SMA (myoepithelial cells) in mice fed a regular or high AGE diet. Scale bar = 20 µM. Inset shows increased magnification of break in myoepithelial cell layer. D-G Representative immunofluorescent images and quantitation of F4/80 (D/E) and vimentin (F/G) surrounding TEB structures in 7 week old mice fed a regular or high AGE diet. Mouse numbers: 7 week—regular (n = 4), low AGE (n = 5), high AGE (n = 5). Values are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.005