Fig. 2

Consumption of a high AGE diet results in abnormal TEB morphology and proliferation. A Representative carmine stained whole mount images of TEBs from 7-week old mice fed regular, low AGE or high AGE diet. Scale bar = 50 µM. Quantification of TEB size (B) and TEB number (C) in 7-week old mice fed regular, low AGE and high AGE diets. Mouse numbers (B-C): regular (n = 4), low AGE (n = 5), high AGE (n = 5). D IHC staining of Ki67 in TEBs from 7-week old mice fed a regular, low AGE and high AGE diet. Inset shows proliferating cells in the stroma surrounding the TEBs in the high AGE fed mice. Scale bar = 50 µM. E Quantitation of Ki67 in TEBs of 7-week old mice fed regular, low AGE and high AGE diet. F Quantitation of Ki67 in the stromal cells surrounding TEBs of 7-week old mice fed regular, low AGE and high AGE diet. Mouse numbers (D-F): regular (n = 3), low AGE (n = 4), high AGE (n = 4). Values are mean ± SEM. *P < 0.05; **P < 0.01