Fig. 7
MIDEAS-AS1 overexpression inhibits tumor formation in nude mice xenograft models. A MDA-MB-231 cells were stably transfected with the MIDEAS-AS1 overexpression plasmid or control plasmid and inoculated subcutaneously into nude mice. Compared with the control plasmid group, MIDEAS-AS1 overexpression inhibited tumor growth. B-C Tumor weight (B) Tumor volume (mm3) (C) were significantly decreased in the MIDEAS-AS1 overexpression group. D Immunohistochemistry with a Ki67-specific antibody was performed in the tumor. The results showed that MIDEAS-AS1 overexpression led to reduced expression of Ki67. Scale bars, 50 μm. E Representative images MATR3, NCALD staining in the tumor. Immunohistochemical staining showed that MIDEAS-AS1 overexpression led to increased expression of NCALD, while MATR3 was not affected. Scale bars, 50 μm. F-G Stably transfected MDA-MB-231 cells were injected into the tail veins of nude mice (n = 5). Representative images of lungs (F) and HE staining of lungs (H) isolated from mice. MIDEAS-AS1 overexpression resulted in a decreased number of lung metastatic colonies (G). I Schematic diagram depicting mechanisms involved in the effect of MIDEAS-AS1. Data were shown as mean ± SD. (*p < 0.05, ** p < 0.01, *** p < 0.001)