Fig. 6

MIDEAS-AS1 exerts its function by regulating the NCALD expression and downstream signaling. A-B The effects of co-transfected with MIDEAS-AS1 overexpression vector or empty vector together with si-NCALD or si-NC on the proliferation of MDA-MB-231 and MDA-MB-468 cells by MTT assay (A) and colony formation assays (B). C-D Transwell and wound-healing assays were used to evaluate the motility of MDA-MB-231 and MDA-MB-468 cells of co-transfected with MIDEAS-AS1 overexpression vector or empty vector together with si-NCALD or si-NC. E Western blot analysis of the corresponding signaling in MDA-MB-231 and MDA-MB-468 cells co-transfected with MIDEAS-AS1 overexpression vector or empty vector together with si-MATR3 or si-NC. Data were shown as mean ± SD. (*p < 0.05, ** p < 0.01, *** p < 0.001)