Fig. 5

The overexpression of NCALD inhibits TNBC cell proliferation and migration in vitro. A-B The efficiency of overexpression and knockdown of NCALD were confirmed by qRT-PCR (A) and Western blot (B) in MDA-MB-231 and MDA-MB-468 cells. C–E The effects of NCALD overexpression and knockdown on the proliferation of MDA-MB-231 and MDA-MB-468 cells were examined by MTT assay (C) and colony formation assays (D-E). F Flow cytometry was performed to measure the effect of NCALD overexpression and knockdown on apoptosis. G Transwell and invasion were used to evaluate the motility of MDA-MB-231 and MDA-MB-468 cells transfected with NCALD-overexpressing vector or control vector (G) and si-NC or si-MIDEAS-AS1 (H). I Western blot analysis of p-ERK1/2, p-NF-κB and TNF-β protein levels in MDA-MB-231 and MDA-MB-468 cells transfected with NCALD overexpression plasmid. J Western blot analysis of p-ERK1/2, p-NF-κB and TNF-β protein levels in MDA-MB-231 and MDA-MB-468 cells transfected with transfected with si-NC or si-NCALD. Data were shown as mean ± SD. (*p < 0.05, ** p < 0.01, *** p < 0.001)