Skip to main content
Fig. 3 | Breast Cancer Research

Fig. 3

From: Long non-coding RNA MIDEAS-AS1 inhibits growth and metastasis of triple-negative breast cancer via transcriptionally activating NCALD

Fig. 3

MIDEAS-AS1 interacts with MATR3. A The expression level of MIDEAS-AS1 in the subcellular fractions of MDA-MB-231 and MDA-MB-468 cells was detected by qRT-PCR, with U6 and GAPDH as nuclear and cytoplasmic markers, respectively. B FISH analysis of the location of MIDEAS-AS1 (red) in the cytoplasm and nuclear fractions of MDA-MB-231 and MDA-MB-468 cells. Scale bars, 20 μm. C Biotin-labeled MIDEAS-AS1 was incubated with MDA-MB-231 cell lysates for pull-down, followed by SDS-PAGE separation, silver staining. D Peptide sequences analysis interacted with MIDEAS-AS1 was performed by mass spectrometry. E Western blot analysis following RNA pull-down assay indicated that MIDEAS-AS1 interacted with MATR3. F RIP assay using Flag antibody showed that MIDEAS-AS1 interacted with MATR3. G Colocalization of MIDEAS-AS1 and MATR3 by immunofluorescence following transfected with MIDEAS-AS1-overexpressing vector or control vector. Scale bars, 20 μm. H The interaction between the truncated MIDEAS-AS1 and MATR3 was confirmed by RNA pull-down and western blot. Data were shown as mean ± SD. (*p < 0.05, ** p < 0.01, *** p < 0.001)

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us