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Fig. 2 | Breast Cancer Research

Fig. 2

From: Long non-coding RNA MIDEAS-AS1 inhibits growth and metastasis of triple-negative breast cancer via transcriptionally activating NCALD

Fig. 2

MIDEAS-AS1 inhibits TNBC cell proliferation and migration in vitro. A–D The effects of MIDEAS-AS1 overexpression and knockdown on the proliferation were examined by MTT assay (A-B) and colony formation assays (C-D). E Flow cytometry was performed to measure the effect of MIDEAS-AS1 on apoptosis. F-G Transwell and invasion assays were used to evaluate the motility of MDA-MB-231 and MDA-MB-468 cells transfected with MIDEAS-AS1-overexpressing vector or control vector (F) and si-NC or si-MIDEAS-AS1 (G). H Western blot analysis of EMT-related proteins in MDA-MB-231 and MDA-MB-468 cells after overexpression of MIDEAS-AS1. I Western blot analysis of EMT-related proteins in MDA-MB-231 and MDA-MB-468 cells after knockdown of MIDEAS-AS1. Data were shown as mean ± SD. (*p < 0.05, ** p < 0.01, *** p < 0.001)

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Breast Cancer Research

ISSN: 1465-542X

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