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Fig. 5 | Breast Cancer Research

Fig. 5

From: Loss of SPRY2 contributes to cancer-associated fibroblasts activation and promotes breast cancer development

Fig. 5

SPRY2 knockdown promotes glycolysis in fibroblasts. A GSEA plot based on gene expression in CAFs compared with NFs using the RNA-seq data from GSE 90505. B Extracellular acidification rate (ECAR) after SPRY2 knockdown in fibroblasts. C, D Lactate production (C) and glucose consumption (D) after SPRY2 knockdown in fibroblasts. E, F. Heatmap and graph showing expression of glycolytic enzymes based on the expression of SPRY2 in GSE10797. G Relative mRNA expression of glycolytic enzymes after SPRY2 knockdown in fibroblasts (normalized with mRNA expression in shNC group). H Relative mRNA expression of fibroblasts activation markers in shNC and shSpry2 knockdown fibroblasts with or without 2-DG treatment (normalized with mRNA expression in shNC group). Data are presented as mean ± SD (n = 3 independent samples). P values based on unpaired Student’s t test: *P < 0.05, **P < 0.01, ***P < 0.001

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Breast Cancer Research

ISSN: 1465-542X

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