Fig. 4

SPRY2 knockdown promotes fibroblast activation in breast cancer stroma. A GSEA and GO analysis of increased expression genes comparing SPRY2-low group and SPRY2-high group by using the RNA sequencing (RNA-seq) data of CAFs from breast cancers in GSE 10797 and GSE9014. B Reactome analysis of Spry2-low group and Spry2-high group by using single-cell-sequencing data of fibroblasts isolated from mammary tumor of MMTV-PyMT mice (GSE111229). C Morphologic changes of fibroblasts after transfected with shRNA targeting SPRY2 or scramble with GFP expressing. Scale bar:100 µm. D mRNA expression of fibroblast activation markers after SPRY2 knockdown. P values based on unpaired Student’s t test: *P < 0.05, **P < 0.01