Fig. 2

Decreased SPRY2 protein expression in stroma predicts a poor prognosis in patients with BRCA. A IHC staining of SPRY2 in a human breast cancer tissue microarray from 132 cases of breast cancer patients. Representative SPRY2 images were shown at left panel. The percentage of tissue cores displaying low and high staining in fibroblasts of normal breast and BRCA lesions was shown at right panel. Scale bar: 50 µm, **P < 0.01. B The correlation between SPRY2 expression in CAFs and patients’ death event, tumor size, AJCC stage and P53 expression was conducted based on the SPRY2 expression in CAFs from the IHC staining of the tissue microarray. C Multivariate Cox regression analysis of SPRY2 expression and clinicopathological factors for overall survival. HR Hazard ratio; CI Confidence interval. D ROC curves of multivariate cox model with or without SPRY2 expression in fibroblasts from IHC staining of human breast cancer tissue microarray