Fig. 6

Downregulation of G3BP2 suppressed BC cell migration and stemness. A The predicted sites of target gene G3BP2 and miR-217 interaction. B Determining luciferase activity after co-transfected miR-217 and vectors with wild type and mutant G3BP2 into MCF-7 cells through dual luciferase assay. C The expression levels of G3BP2 were determined in MCF-7 and MDA-MB-231 cells after PBS-EXO or PTX-EXO treatment. D The G3BP2 levels were detected by RT-PCR in MCF-7 cells transfected with vectors or circBACH1 containing vectors, vetors transfection was set as control. E Western blot assay was performed to determine the G3BP2 proteins levels in MCF-7 cells transfected with circBACH1 or miR-217 vectors. F The G3BP2 protein levels were measured by western blotting assay after downregulation of G3BP2 mRNA. G, H Determination of the migrated cell number of MCF-7 cells transfected with si-G3BP2 by Transwell assay, scale bar: 25 µm. I, J Mammosphere formation (%) was determined in MCF-7 cells treated with si-G3BP2, scale bar: 50 µm. K The G3BP2 expression was detected by immunohistochemistry in tissue of BC patients, using normal tissue as control (×400). L The G3BP2 expression was measured in tissue of BC patients by RT-PCR, using normal tissue as control. *P < 0.05, **P < 0.01, ***P < 0.001