Fig. 5

miR-217 was interacted with circBACH1 in BC cells. A The Venn diagram of miRNAs predicted to interact with circBACH1 from three database such as circbank, NIH, ENCORI was drawn. B The predicted sites of miR-217 and circBACH1 interaction and the mutated sites of circBACH1. C The expression of miR-217 was determined by RT-PCR in MCF-7 and MDA-MB-231 cells after PBS-EXO or PTX-EXO treatment. D Determining luciferase activity in MCF-7 cells after co-transfected with miR-217 and vectors with wild type and mutant circBACH1 through dual luciferase assay. E, F The amount of circBACH1 and miR-217 were pulled down by Ago2-RIP assay in MCF-7 cells after treated with miR-217 inhibitors or control. CircBACH1 and miR-217 expression levels were measured by RT-PCR. G, H The expression of miR-217 in MCF-7 cells was examined by RT-PCR after transfected with vectors or circBACH1 containing vectors (G), si-NC or si-circBACH1 (H), vetors or si-NC transfection was set as control. I miR-217 expression was measured in the tissue of BC patients by RT-PCR, using normal tissue as control. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ##P < 0.01, ###P < 0.001, compared with the control group