Fig. 4

Downregulation of circBACH1 inhibited the PTX-EXO-induced tubes formation of HUVECs. A, C The MCF-7 and MDA-MB-231 cell migration was detected after treated with PTX and PBS-EXO or PTX-EXO and si-circBACH1, scale bar: 25 µm. The migrated cell number of MCF-7 (B) and MDA-MB-231 (D) was calculated after treated with PTX and PBS-EXO or PTX-EXO, or si-circBACH1. E Representative micrographs of HUVECs forming the tube structures in vitro after 24 h. F Quantitative analysis of the tube lengths of HUVECs following treatment with MCF-7 medium after treated with PTX and PBS-EXO or PTX-EXO, or and si-circBACH1. ***P < 0.001, compared with the PTX + PBS-EXO group. ##P < 0.01, ###P < 0.001, compared with the PTX + PTX-EXO group