Fig. 3

Downregulation of circBACH1 reversed the PTX-EXO-induced PTX-resistance of BC cells. A, B Cell viability were tested by CCK8 in MCF-7 (A) and MDA-MB-231 (B) treated with PTX, accompanied exosomes separated from BC cell lines with PBS treatment (PBS-EXO) or with PTX treatment (PTX-EXO), or circBACH1 inhibitor (si-circBACH1). C, D Stemness markers (CD44, CD133, Oct) of BC were measured by western blotting in MCF-7 (C) and MDA-MB-231 (D) treated with PTX, PBS-EXO or PTX-EXO, or si-circBACH1, and β-actin was set as control. E, F The expression of stemness markers in MCF-7 (E) and MDA-MB-231 (F) after transfected with vectors, circBACH1 containing vectors, si-NC, or si-circBACH1 were detected by western blotting, vetors or si-NC transfection was set as control. ***P < 0.001