Fig. 2

CircBACH1 was mainly located in the cytoplasm of BC cells. A The location of circBACH1 in MCF-7 was determined by Fluorescence in Situ Hybridization (FISH) assay, scale bar: 50 µm. B The expression of circBACH1 was measured in the nucleus and cytoplasm of MCF-7. C CircBACH1 expression was measured by RT-PCR in human normal mammary epithelial cells (MCF-10A) and BC cells (MCF-7 and MDA-MB-231) after PBS or PTX treatment. D CircBACH1 expression was measured in tissue of BC patients, using normal tissue as control. E Northern blotting of circBACH1 expression by transfection with si-circBACH1. F, H Mammosphere formation (%) was determined in MDA-MB-231 and MCF-7 cells treated with PTX, PBS-EXO or PTX-EXO, or si-circBACH1, scale bar: 50 µm. G Analysis of CD44 and CD24 expression in MCF-7 cells treated with PTX, PBS-EXO or PTX-EXO, or si-circBACH1 by flow cytometry. I The proportion of CD44⁺ CD24⁻ cells were quantified in PTX, PBS-EXO or PTX-EXO, or si-circBACH1 treated cells. *P < 0.05, ***P < 0.001