Fig. 1

CircBACH1 was highly expressed in the exosomes derived from PTX-treated BC cells. A The exosomes were identified by transmission electron microscopy. B The surface markers of exosomes were detected by western blotting pre- or post- PTX treatment. C Volcano plot of the differentially expressed circRNAs. Red represents up-regulated circRNAs, and green represents down-regulated circRNAs. The vertical lines demark the fold change values, which correspond to 2.0-fold up and down. The horizontal line marks a P value of 0.05; D A heat map of cluster analysis demonstrating the different circRNA expression profiles between four gorups of exosomes derived from PTX-treated normal mammary epithelial (MCF-10A) cells (C1, C2, C3, and C4) and six gorups of exosomes derived from PTX-treated MCF-7 cells (M1, M2, M3, M4, M5 and M6). The intensity is reflected in the color scale that runs from green (low intensity) to red (high intensity). E CircBACH1 expression was measured by RT-PCR in the exosomes derived from human normal mammary epithelial cells (MCF-10A) and BC cells (MCF-7 and MDA-MB-231) after PBS or PTX treatment. F The expression of circBACH1 in MCF-7 and MDA-MB-231 was examined after incubation with PBS-EXO (the exosomes of BC cells after PBS treatment) or PTX-EXO (the exosomes of BC cells after PTX treatment). G CircBACH1 expression was examined in the plasma exosomes of BC patients pre- or post- PTX treatment. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001