Fig. 8

Mutant SF3B1-R625C, H19-S, and Let-7a mediate the malignant transformation of I-AT2 cells. A The lung metastases (n = 20) of MPG-ST-iDTR mice 2 weeks after DT injection were collected and sent for Sanger sequence traces of SF3B1-wild-type and SF3B1-mutant tumors. Representative mutations were showed. n = number of lung metastases. B N-AT2, I-AT2, and M-AT2 cells were transfected with the expression vectors of SF3B1-WT, SF3B1-mut (R625G) or their controls. The expression of H19-S or H19-L was analyzed by qRT-PCR. C. N-AT2, I-AT2 and M-AT2 cells were transfected with SF3B1 siRNAs or their control siRNAs. The expression of SF3B1 (a) or H19-S (b) was individually detected. Abbreviations: C, Control; WT, SF3B1-WT; mut, SF3B1-mut; SiCtr, SiRNA control; Si-4#, SF3B1 SiRNA-4#; Si-5#, SF3B1 SiRNA-5#; N.S, no significance; N-AT2, normal AT2 cells; I-AT2, immortalized AT2 cells; M-AT2, malignant AT2 cells; pH19-L, full-length mouse H19; pH19-S H19 without exon 2, i.e., the “sponge” in this experiment. *P < 0.05