Fig. 6

H19-S promotes tumorigenicity in immortalized AT2 cells. The tdTomato-positive AT2 cells were sorted from the peritumoral tissues (normal AT2 cells, n = 3) or tumor tissues (immortalized AT2 cells, n = 15) of the lung metastasis from 16-week-old MPG-ST-iDTR mice without DT injection. n = number of tissues. The plasmids including H19-L (full-length transcript) or H19-S (short transcript) were separately transfected into immortalized AT2 or normal AT2 cells. A qRT-PCR assessment of H19-L and H19-S in H19-L– or H19-S–overexpressing immortalized AT2 cells, normal AT2 cells, and their control cells. Data presented are the mean of triplicate experiments. Error bars indicate standard deviation (**P < 0.01; *P < 0.05; N.S, No significance). B Invasive capacities of the indicated cells were analyzed and quantified. Data presented are the mean of triplicate experiments. Error bars indicate standard deviation (**P < 0.01; N.S, No significance). C Colony-forming assays of H19-L or H19-S stably overexpressed in immortal AT2 cells, normal AT2 cells, and their control cells. Data presented are the mean of triplicate experiments. Error bars indicate standard deviation (**P < 0.01; N.S, No significance). D H19-L or H19-S stably overexpressed immortal AT2 cells, normal AT2 cells, and their control cells were subcutaneously transplanted into wild-type (WT) recipient mice. The tumor incidence, described as transplantation sites with tumors/transplanted mice amount (ratio), were measured at 18 weeks after transplantation. Abbreviations: C, plasmid control; OE-S, overexpression of H19-S; OE-L, overexpression of H19-L