Fig. 2

T2-weighted imaging and tumor volumetry after targeted therapy. Treatment initiation of both targeted therapies was started on day three (3d) after tumor implantation. On day six (6d) or day nine (9d), sorafenib and ICI-treated tumors were compared to untreated controls. a Size of 67NR tumors decreased after both sorafenib treatment and immune checkpoint blockade on day six and day nine, with small areas of intratumoral necrosis (white arrows). Tumor volumes of 67NR tumors before treatment initiation on day three are displayed in gray. b Sorafenib treatment caused no significant changes in volumes of 4T1 tumors both on day six and day nine. After treatment, tumors developed circumscribed necrosis, specifically arranged in the tumor center. ICI-treated 4T1 tumors showed increased tumor volumes compared to control groups and exhibited excessive intratumoral necrosis. Tumor volumes of 4T1 tumors before treatment initiation on day three are displayed in gray. Each dot represents one animal, with horizontal lines indicating the mean. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001