Fig. 4

Vangl localizes to the migratory protrusions of single migratory cells and the leading edge of leader cells in collectively migrating breast cancer cells and promotes a hyper-protrusive morphology. a Representative confocal images of migratory MDA-MB-231 cells stained for Vangl1:green or Vangl2:green, Actin: gray, and DAPI: blue. b Representative confocal images of collectively migrating MCF7-Vector cells stained for Vangl1: green, Actin: gray, and DAPI: blue, MCF7-Flag-Vangl1 cells stained for Flag: green, Actin: gray, and DAPI: blue, and MCF7-V5-Vangl2 cells stained for V5: green, Actin: gray, and DAPI: blue, yellow arrows: Vangl-rich protrusions, scale bar = 10 µm