Fig. 4
PKC-ζ regulated TGF-β1EV secretion in 4T1 cells. A, B 4T1 cells were treated with 20, 50, 100 ng/mL PMA or CAL for 48 h. EVs and EV-associated TGF-β1 were quantified by BCA (A) and ELISA (B), respectively. C Relative mRNA expression level in responding to different dose of X-Ray. D, E 4T1 cells were transfected with PKC-ζ siRNA for 24 h followed by treatment with PMA (100 ng/mL) for 48 h. EVs and EV-associated TGF-β1 were quantified by BCA (D) and ELISA (E), respectively. F, G 4T1 cells were treated by 2–8 Gy X-Ray. EVs and EV-associated TGF-β1 were quantified by BCA (F) and ELISA (G), respectively. H, I 4T1 cells were transfected with PKC-ζ siRNA for 24 h followed by treatment with 8 Gy X-Ray. EVs and EV-associated TGF-β1 were quantified by BCA (H) and ELISA (I), respectively. J 4T1 cells were treated by 2–8 Gy X-Ray. Cells were collected and cell lysate were detected by western blot for PKC-ζ and p-PKC-ζ. *p < 0.05; **p < 0.01. All experiments was analyzed by 2-way ANOVA