Fig. 5

CMTM7 suppressed Wnt signaling by inhibiting the nuclear localization of β-catenin. A The GO analysis was performed among the genes identified through mass spectrometry. The TOP/FOP luciferase reporter assay in CMTM7-overexpressed MCF-7 cells (B) and in CMTM7-depleted MDA-MB-231 cells (C). D The localization transformation of β-catenin visualized by IF. E The β-catenin expression level in the nucleus and cytoplasm detected by nucleocytoplasmic separation western blot. F The expression levels of downstream target genes of Wnt/β-catenin signaling, including cyclin D1, survivin, c-myc, and β-catenin, detected by western blot. G The target genes expression after treatment of Wnt/β-catenin signaling inhibitor adavivint in CMTM7-depleted MDA-MB-231 cells. H The localization of β-catenin in CMTM7-overexpressed and CTNNA1-depleted MCF-7 cells detected by IF. *p < 0.05; ***p < 0.01