Fig. 2

Mebendazole induces G2/M cell cycle arrest and apoptosis. A Expression of proteins involved in G2/M cell cycle arrest was analyzed by Western blot using lysate extracted from MDA-MB-231 (left) and SUM159 (right) cell lines treated with increasing doses of MBZ as indicated. B The percentage of MDA-MB-231 cells in each phase of cell cycle was determined after treating the cells for 48 h with MBZ at the indicated doses followed by propidium iodide staining and flow cytometry analysis. Mean ± SEM; N = 3 independent experiments. P values for two-way ANOVA test *< 0.05, **< 0.01,***< 0.001,****< 0.0001. C Expression of proteins involved in MBZ-induced apoptosis was analyzed by Western blot using lysate extracted from MDA-MB-231 and SUM159 cells treated with increasing doses of MBZ for 72 h