Fig. 4

Paqr8 enhances cell survival without affecting proliferation following Her2 downregulation. Regressing tumors from nu/nu mice bearing Paqr8-OE or Paqr8-KO primary orthotopic tumors at three days (D3) following Her2 downregulation were stained by immunofluorescence for cleaved caspase-3 (cc3). Representative images are shown (A, B), and the proportions of eGFP + cells that were cc3 + were quantified (C, D). D3 regressing tumors were also stained for EdU and Ki67, and the proportions of eGFP + cells that were EdU + or Ki67 + were quantified (E, F). Paqr8-OE (G, I) or Paqr8-KO (H, J) cells were cultured in medium containing 1% serum without doxycycline (Her2 OFF) for 72 h. Cells were incubated with 10 mM EdU for 2 h prior to fixation, permeabilization, and staining by immunofluorescence. G, H Quantification of the proportion of eGFP + cells that were cc3+. I, J Quantification of the proportion of eGFP + cells that were EdU + or Ki67+