Fig. 3

Paqr8 confers a competitive advantage to tumor cells following Her2 downregulation. A Experimental schema for in vivo and in vitro competition assays. B eGFP to mCherry ratios determined by ddPCR after injecting a mixture of 80% H2B-eGFP-labeled Paqr8-OE cells and 20% H2B-mCherry-labeled control cells. C eGFP to mCherry ratios determined by ddPCR after injecting a mixture of 20% eGFP-labeled Paqr8-KO and 80% mCherry-labeled sgRosa control cells. D eGFP to mCherry ratios determined by ddPCR after plating 50% H2B-eGFP-labeled Paqr8-OE cells and 50% H2B-mCherry-labeled control cells, following Her2 downregulation at time D0. Data were normalized to a control arm for which 50% H2B-eGFP-labled control cells and 50% H2B-mCherry-labeled control cells were plated in parallel. E eGFP to mCherry ratios determined by ddPCR from plating 50% eGFP-labeled sgPaqr8 cells and 50% mCherry-labeled sgRosa control cells, following Her2 downregulation at time D0. Data were normalized to a control arm for which 50% eGFP-labeled sgRosa cells and 50% mCherry-labeled sgRosa cells were plated in parallel