Fig. 2
From: PAQR8 promotes breast cancer recurrence and confers resistance to multiple therapies
Paqr8 is necessary and sufficient for efficient tumor recurrence and colony formation following Her2 downregulation. A Schematic showing recurrence-free survival assays following orthotopic injection of Her2-dependent primary mouse tumor cells. Kaplan–Meier curves depicting rate of recurrence following Her2 downregulation comparing (B) Her2-dependent primary tumor cells overexpressing Paqr8 (Paqr8-OE) and controls, or (C) Her2-dependent primary tumor cells deleted for Paqr8 using CRISPR-cas9 and two independent guide RNAs (sgPaqr8_gA, sgPaqr8_gB) and controls (sgRosa). Colony formation assays performed in medium containing 10% serum with doxycycline (Her2 ON) or in medium containing 1% serum without doxycycline (Her2 OFF), comparing (D) Her2-dependent primary tumor cells overexpressing Paqr8 and controls, or (E) cells deleted for Paqr8 and sgRosa controls. F, G Bar graphs quantifying the proportion of plated cells that formed colonies under Her2 ON and OFF conditions. H Viable cell counts for BT474-M1 cells in medium containing lapatinib or vehicle control (DMSO)