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Fig. 1 | Breast Cancer Research

Fig. 1

From: Upregulation of Nox4 induces a pro-survival Nrf2 response in cancer-associated fibroblasts that promotes tumorigenesis and metastasis, in part via Birc5 induction

Fig. 1

GKT137831 suppressed the activated phenotype of mammary fibroblasts. (A) Fibroblasts were treated with GKT137831 at indicated doses for 48 h prior to reseeding in collagen matrix. Area of the collagen discs were measured after 16 h of contraction. Representative results of 3 independent contraction studies are shown here. Error bars = standard deviation of N = 3 independent studies. (B) GKT137831 effectively reduced cellular oxidative stress, as demonstrated by the levels of H2O2 released into the cell culture media via AmplexRed assay. DPI was used as a pan-Nox inhibitor. Error bars = standard deviation of N = 3 independent studies. (C) RMF-HGF that was pre-treated with GKT137831 for 24 h was seeded in a Matrigel invasion lower-well as a chemoattractant and invasion of MDA-MB231 cells was determined after 16 h of seeding. Error bars = standard deviation of N = 6 independent experiments. (D) Real-time PCR analysis showing mRNA expression of some key myofibroblast markers in GKT137831 treated RMF-HGF

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Breast Cancer Research

ISSN: 1465-542X

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