Skip to main content
Fig. 2 | Breast Cancer Research

Fig. 2

From: Combined Dusp4 and p53 loss with Dbf4 amplification drives tumorigenesis via cell cycle restriction and replication stress escape in breast cancer

Fig. 2

Enhanced soft agar growth with combined Dusp4 loss and Trp53 (p53) deletion. A Crystal violet 2D culture assay of PMECs transduced with CRISPR/Cas9 constructs targeting Trp53 (exon 7 or exon 5) or scramble control (Parent) and selected with 10uM nutlin-3a for 1 week, or DMSO control. Trp53 CRISPR transductants demonstrate insensitivity to MDM2 inhibition, verifying loss of p53 function. B Soft agar colony formation assay for Dusp4FLOX or Dusp4NULL PMECs transduced with control sgRNA or Trp53 sgRNA. C Western blot analysis of derivative PMECs

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us